Keywords

biofilm, outer membrane permeability, Pseudomonas aeruginosa, ultrasound

Abstract

Low-frequency ultrasound has been investigated as an adjuvant to antimicrobial therapy, targeted at both planktonic and biofilm (sessile) organisms. Our previous work showed that ultrasound (US) effectively enhances the bactericidal activity of certain antibiotics against planktonic cultures (Pitt et al., 1994; Rediske et al., 1999) and in vitro biofilms (Johnson et al., 1998; Qian et al., 1999) and in vivo biofilms (Carmen et al., 2004b, 2005; Rediske et al., 2000) of gram-positive and gram-negative bacteria. Ultrasound was shown to increase the transport of antibiotics through biofilms (Carmen et al., 2004a) which could account for some (or all) of the enhanced antibiotic activity against insonated biofilms; but such a mechanism could not account for US-enhanced antibiotic activity in planktonic cultures which have no extensive exopolymer matrix to retard antibiotic transport.

Original Publication Citation

Christopher M. Runyan, John C. Carmen, Benjamin L. Beckstead, Jared L. Nelson, Richard A. Robison, William G. Pitt, Low-frequency ultrasound increases outer membrane permeability of Pseudomonas aeruginosa, The Journal of General and Applied Microbiology, 2006, Volume 52, Issue 5, Pages 295-301, Released on J-STAGE February 20, 2007, Online ISSN 1349-8037, Print ISSN 0022-1260, https://doi.org/10.2323/jgam.52.295, https://www.jstage.jst.go.jp/article/jgam/52/5/52_5_295/_article/-char/en

Document Type

Peer-Reviewed Article

Publication Date

2006-09-20

Publisher

MDPI

Language

English

College

Ira A. Fulton College of Engineering

Department

Chemical Engineering

University Standing at Time of Publication

Full Professor

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