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Keywords

bone marrow, hematopoiesis, immune cell development, lineage markers

Abstract

The Mafia mouse model expresses the green fluorescent protein (GFP) in cells that utilize the macrophage-specific c-fms promoter. The c-fms promoter controls expression of receptor for Colony Stimulating Factor 1. We hypothesize that this promotor may be utilized in bone marrow progenitor cells in addition to the mature cell expression already known in macrophage lineage cells. For this study, bone marrow cells were analyzed by flow cytometry for the expression of c-fms, early progenitor markers, and lineage-specific markers. Expression was also mapped on a Ly6CxCD31 plot, which has been used to distinguish cells based on developmental stage and lineage. This was used to visualize the developmental pathways of the bone marrow. Experimental findings provide a clearer framework for tracking hematopoiesis and specific lineage progression and confirm the utility of c-fms as a developmental marker in conjunction with the Ly6CxCD31 plot.

Document Type

Poster

Publication Date

2026-03-26

Language

English

College

Life Sciences

Department

Microbiology and Molecular Biology

University Standing at Time of Publication

Senior

C-FMS Expresses in Early Progenitor Cells & Myeloid-Lineage Leukocytes Based on Analyzing Lineage Markers and Mapping on Ly6C x CD31 Plots in Bone Marrow

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Life Sciences Commons

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