Keywords
cell-free protein synthesis (CFPS), linear DNA expression templates (LET), high-throughput protein screening
Abstract
A rapid, versatile method of protein expression and screening can greatly facilitate the future development of therapeutic biologics, proteomic drug targets and biocatalysts. An attractive candidate is cell-free protein synthesis (CFPS), a cell-lysate-based in vitro expression system, which can utilize linear DNA as expression templates, bypassing time-consuming cloning steps of plasmid-based methods. Traditionally, such linear DNA expression templates (LET) have been vulnerable to degradation by nucleases present in the cell lysate, leading to lower yields. This challenge has been significantly addressed in the recent past, propelling LET-based CFPS as a useful tool for studying, screening and engineering proteins in a high-throughput manner. Currently, LET-based CFPS has promise in fields such as functional proteomics, protein microarrays, and the optimization of complex biological systems.
Original Publication Citation
Song-Min Schinn, Andrew Broadbent, William T. Bradley, Bradley C. Bundy, Protein synthesis directly from PCR: progress and applications of cell-free protein synthesis with linear DNA, New Biotechnology, Volume 33, Issue 4, 2016, Pages 480-487, ISSN 1871-6784, https://doi.org/10.1016/j.nbt.2016.04.002.
BYU ScholarsArchive Citation
Schinn, Song-Min; Broadbent, Andrew; Bradley, William T.; and Bundy, Bradley Charles, "Protein synthesis directly from PCR: progress and applications of cell-free protein synthesis with linear DNA" (2016). Faculty Publications. 7838.
https://scholarsarchive.byu.edu/facpub/7838
Document Type
Peer-Reviewed Article
Publication Date
2016-04-13
Publisher
New Biotechnology
Language
English
College
Ira A. Fulton College of Engineering
Department
Chemical Engineering
Copyright Status
2016 Elsevier B.V. All rights reserved.
Copyright Use Information
https://lib.byu.edu/about/copyright/