Keywords

Capsid, Enzyme immobilization, Virus-like particle, Virus-based nanoparticle

Abstract

Enzyme-mediated biocatalysis is generally more selective and environmentally friendly and requires less energy than chemocatalysis. However, factors such as temperature, acidity and the presence of proteases can negate enzyme activity. Encapsulation in virus-like particles is one promising method to mitigate these difficulties. Encapsulation also can be used to create multi-reaction nanoreactors that increase process efficiency by isolating reaction intermediates. To successfully encapsulate enzymes, a variety of methods involving both non-covalent and covalent interactions have been developed. Here we review promising virus-like particle encapsulation strategies, their advantages and remaining challenges.

Original Publication Citation

Joshua W. Wilkerson, Seung-Ook Yang, Parker J. Funk, Steven K. Stanley, Bradley C. Bundy, Nanoreactors: Strategies to encapsulate enzyme biocatalysts in virus-like particles, New Biotechnology, Volume 44, 2018, Pages 59-63, ISSN 1871-6784, https://doi.org/10.1016/j.nbt.2018.04.003.

Document Type

Peer-Reviewed Article

Publication Date

2018-04-24

Publisher

New Biotechnology

Language

English

College

Ira A. Fulton College of Engineering

Department

Chemical Engineering

University Standing at Time of Publication

Full Professor

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