Keywords
in vitro transcription/translation, cell-free protein synthesis, lyophilization, cell-extract preparation, cell-free energy system, GFP reporter protein
Abstract
Cell-free protein synthesis (CFPS) is a versatile tool for rapid recombinant protein production and engineering. One drawback of cell-free technology is the necessity to store the major components—cell extracts and energy systems—below freezing in bulky aqueous solutions. Here we describe simple methods for lyophilizing extracts and preparing powdered energy systems for CFPS. These techniques allow for high-density storage of cell-free systems that are more robust against temperature and bacterial degradation. Our methods have the potential to decrease storage expenses, allow for longer shelf-life of cell extracts at room temperature, and enable durable portable protein production technologies.
Original Publication Citation
Smith, M. T., Berkheimer, S. D., Werner, C. J., & Bundy, B. C. (2014). Lyophilized Escherichia Coli-Based Cell-Free Systems for Robust, High-Density, Long-Term Storage. BioTechniques, 56(4), 186–193. https://doi.org/10.2144/000114158
BYU ScholarsArchive Citation
Smith, Mark Thomas; Berkheimer, Scott D.; Werner, Christopher J.; and Bundy, Bradley Charles, "Lyophilized Escherichia Coli-Based Cell-Free Systems for Robust, High-Density, Long-Term Storage" (2018). Faculty Publications. 7829.
https://scholarsarchive.byu.edu/facpub/7829
Document Type
Peer-Reviewed Article
Publication Date
2018-04-03
Publisher
BioTechniques
Language
English
College
Ira A. Fulton College of Engineering
Department
Chemical Engineering
Copyright Status
© 2014 Author(s)
Copyright Use Information
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