Keywords
Blood; Bacteria, Separation, Filtration, Rapid Diagnostics
Abstract
Blood stream infections (BSIs) are challenging to rapidly and precisely diagnose. Current clinical diagnostic methods for BSI identification require culturing the blood sample for many hours prior to identifying the bacteria and possible antibiotic resistance. Removing the culturing step from the bacterial identification process of a BSI provides a significant reduction in the processing time, but shifts the rate-limiting step from the growth time to the concentration procedure, since CFU levels in clinical blood samples can be as low as 10 CFU/mL in blood. This study developed a novel whole blood filtration method to concentrate bacteria from a BSI without culturing the blood or bacteria. Filtering whole blood achieved 100% bacterial removal from 5mL of whole blood in ~90 s, but the bacteria were difficult to remove from the filter. Bacterial removal from the filter after blood filtration was also investigated. At high laboratory loadings of bacteria into freshly collected human blood, a blood lysis solution of 3% Tween 80 followed by a 3% Pluronic F108 backflush solution achieved 80% removal of the bacteria from the filter. This is a major step in collection of bacteria directly from blood for rapid diagnostics without a culture amplification step and with a single backflush.
Original Publication Citation
Ryan L. Wood, Rebecca Prymak, Evelyn Welling, Alayna Bedwell, Jordan T. Wood, Damian Teasley, Magdalena Crofts, Hope Callister, Clifton Anderson, Danielle Glasgow, W. Cameron Beard, Jacob Stepan, Alexandra Carter, William G. Pitt*, “Bacterial Extraction and Concentration from Blood through Filtration Processes”, Am. J. Biomed. Sci. Res., 19(6), 2569 (2023). DOI: 10.34297/AJBSR.2023.19.002659
BYU ScholarsArchive Citation
Wood, Ryan L.; Prymak, Rebecca; Welling, Evelyn; Bedwell, Alayna; Wood, Jordan T.; Teasley, Damian E.; Crofts, Magdalena; Anderson, Hope Callister; Anderson, Clifton M.; Beard, W Cameron; Stepan, Jacob; Carter, Alexandra; and Pitt, William G., "Bacterial Extraction and Concentration from Blood through Filtration Processes" (2023). Faculty Publications. 7715.
https://scholarsarchive.byu.edu/facpub/7715
Document Type
Peer-Reviewed Article
Publication Date
2023-08-28
Publisher
BiomedGrid
Language
English
College
Ira A. Fulton College of Engineering
Department
Chemical Engineering
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