Keywords

Micellar drug delivery, Pluronic, flow cytometry, ultrasound

Abstract

Background

This paper examines the mechanism of ultrasonic enhanced drug delivery from Pluronic micelles. In previous publications by our group, fluorescently labeled Pluronic was shown to penetrate HL-60 cells with and without the action of ultrasound, while drug uptake was increased with the application of ultrasound.

Methods

In this study, the amount of uptake of two fluorescent probes, Lysosensor Green (a pH-sensitive probe) and Cell Tracker Orange CMTMR (a pH-independent probe), was measured in HL-60 and HeLa cells.

Results

The results of our experiments show that the increase in drug accumulation in the cells as a result of ultrasonication is not due to an increase in endocytosis due to ultrasonication.

Conclusions

We hypothesize that sonoporation plays an important role in the acoustically activated drug delivery of chemotherapy drugs delivered from Pluronic micelles.

Original Publication Citation

Husseini, G.A., Runyan, C.M., and Pitt*, W.G., “Investigating the Mechanism of Acoustically Activated Uptake of Drugs from Pluronic Micelles”, BMC Cancer, 2(1), 20-25 (2002). http://www.biomedcentral.com/content/pdf/1471-2407-2-20.pdf

Document Type

Peer-Reviewed Article

Publication Date

2002-08-30

Publisher

Springer Nature

Language

English

College

Ira A. Fulton College of Engineering

Department

Chemical Engineering

University Standing at Time of Publication

Full Professor

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