The Comet Assay to Determine the Mode of Cell Death for the Ultrasonic Delivery of Doxorubicin to Human Leukemia (HL-60 cells) from Pluronic P105 Micelles

Authors

Ghaleb A. Husseini, American University of Sharjah
Kim L. O'Neill, Brigham Young University
William G. Pitt, Brigham Young University

Keywords

Micellar drug delivery, Doxorubicin, Comet Assay, Ultrasound, Apoptosis

Abstract

This notes examines the mode of cell death of HL-60 cells exposed to 70 kHz and 1.3 W/cm2 in the presence of 1% Pluronic P105 and 1.67 μg/ml doxorubicin (Dox). The cells were ultrasonicated for 30, 60 and 120 minutes. They were then lysed, electrophorised, stained using propidium iodide, and their DNA profile captured using a fluorescent microscope. The gradual DNA damage observed and the comet tails captured after 1 and 2 hours of insonation suggest that the mode of cell killing is apoptosis.

Original Publication Citation

Husseini, G.A., O’Neill, K.L., and Pitt*, W.G., “The Comet Assay to Determine the Mode of Cell Death for the Ultrasonic Delivery of Doxorubicin to Human Leukemia (HL-60 cells) from Pluronic P105 Micelles”, Technol. Cancer Res. Treatment, 4(6), 707-712 (2005).

BYU ScholarsArchive Citation

Husseini, Ghaleb A.; O'Neill, Kim L.; and Pitt, William G., "The Comet Assay to Determine the Mode of Cell Death for the Ultrasonic Delivery of Doxorubicin to Human Leukemia (HL-60 cells) from Pluronic P105 Micelles" (2006). Faculty Publications. 7671.
https://scholarsarchive.byu.edu/facpub/7671

Document Type

Peer-Reviewed Article

Publication Date

2006-02-10

Publisher

Sage

Language

English

College

Ira A. Fulton College of Engineering

Department

Chemical Engineering

University Standing at Time of Publication

Full Professor

Copyright Use Information

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