Pad Printing of Carbon Electrodes with Argon Plasma Activation as a Simple and Low Temperature Manufacturing Process for Antibody-Type Biosensors
Keywords
biosensors, carbon electrodes, electrochemical impedance spectroscopy, functionalization, immunosensors, near-patient testing, pad printing
Abstract
In diagnostic tools, rapid in vitro tests such as COVID-19 antigen or pregnancy tests are gaining significance for identifying various pathologies or health conditions. This shift contributes to a change in the way diagnostic efforts are carried out, emphasizing decentralized approaches that offer valuable services within communities, yielding long-term advantages for the healthcare system. Considering the substantial quantity of these tests manufactured and used annually, a straightforward manufacturing process is proposed for highly sensitive carbon electrodes designed for antibody-type biomarker sensors. This process, utilizing pad printing – an additive, low-temperature, and cost-effective method, coupled with plasma activation – has proven the electrodes capability to measure interferon gamma protein, a tuberculosis biomarker. Using electrochemical impedance spectroscopy, the electrodes display high sensitivity and are capable of measuring concentrations from 10 to 1000 pg mL−1 in undiluted serum within an hour. The sensor, utilizing solely a monolayer of antibodies, achieves a performance equivalent to that of a commercial standard sandwich ELISA tested in this study.
Original Publication Citation
Condemi, E., Kunikowski, J., Schoinas, S., Passeraub, P., "Pad Printing of Carbon Electrodes with Argon Plasma Activation as a Simple and Low Temperature Manufacturing Process for Antibody-Type Biosensors," Advanced Sensor Research, Vol. 3, No. 9, 2024
BYU ScholarsArchive Citation
Condemi, Enrico; Kunikowski, Joanna; Schoinas, Spyridon; and Passeraub, Philippe, "Pad Printing of Carbon Electrodes with Argon Plasma Activation as a Simple and Low Temperature Manufacturing Process for Antibody-Type Biosensors" (2024). Faculty Publications. 7561.
https://scholarsarchive.byu.edu/facpub/7561
Document Type
Peer-Reviewed Article
Publication Date
2024-05-30
Publisher
Wiley-VCH GmbH
Language
English
College
Ira A. Fulton College of Engineering
Department
Manufacturing Engineering
Copyright Status
© 2024 The Author(s). Advanced Sensor Research published by Wiley-VCH GmbH. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Copyright Use Information
https://lib.byu.edu/about/copyright/