carbon nanotubes, chemical vapor deposition, point-of-care diagnostics, immunosensor, cancer, CIP2A
Vertically aligned carbon nanotube array (VANTA) coatings have recently garnered much attention due in part to their unique material properties including light absorption, chemical inertness, and electrical conductivity. Herein we report the first use of VANTAs grown via chemical vapor deposition in a 2D interdigitated electrode (IDE) footprint with a high height-to-width aspect ratio (3:1 or 75:25 µm). The VANTA-IDE is functionalized with an antibody (Ab) specific to the human cancerous inhibitor PP2A (CIP2A)—a alivary oncoprotein that is associated with a variety of malignancies such as oral, breast, and multiple myeloma cancers. The resultant immunosensor is capable of detecting CIP2A label-free across a wide linear sensing range (1 – 100 pg/mL) with a detection limit of 0.24 pg/mL within saliva supernatant—a range that is more sensitive than the corresponding CIP2A enzyme linked immunosorbent assay (ELISA). These results help pave the way for rapid cancer screening tests at the point-of-care (POC) such as for the early-stage diagnosis of oral cancer at a dentist's office.
Original Publication Citation
Ding, S., Das, S. R., Brownlee, B. J., Parate, K., Davis, T. M., Stromberg, L. R., Chan, E. K. L., Katz, J., Iverson, B. D., and Claussen, J., 2018, "CIP2A immunosensor comprised of vertically- aligned carbon nanotube interdigitated electrodes towards point-of-care oral cancer screening," Biosensors and Bioelectronics, Vol. 117, pp. 68-74. DOI: 10.1016/j.bios.2018.04.016
BYU ScholarsArchive Citation
Ding, Shaowei; Das, Suprem R.; Brownlee, Benjamin J.; Parate, Kshama; Davis, Taylor; Stromberg, Loreen R.; Chan, Edward K.L.; Katz, Joseph; Iverson, Brian D.; and Claussen, Jonathan C., "CIP2A Immunosensor Comprised of Vertically-aligned Carbon Nanotube Interdigitated Electrodes Towards Point-of-Care Oral Cancer Screening" (2018). All Faculty Publications. 2975.
Ira A. Fulton College of Engineering and Technology
© 2018 Elsevier B.V. All rights reserved. This is the author's submitted version of this article. The definitive version can be found at https://www.sciencedirect.com/science/article/pii/S0956566318302768?via%3Dihub
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Available for download on Tuesday, October 15, 2019