Abstract
Resistant (65-410 and 65-393) and susceptible (breeders Lahontan) alfalfa seedlings were infected with the Northern root-knot nematode, Meloidogyne hapla. Infected roots were fixed, stained, and embedded in plastic for ultrastructural investigations. Sections of plastic embedded tissue, cut at 0.5-1.5 microns for light microscopy investigations, were stained with various histochemical stains to determine the chemical composition of the nematode-altered tissues. In the susceptible cultivar, giant cells were formed by enlargement of nematode-stimulated cells without cell wall lysis. The giant cells became multinucleate by karyonkinesis without cytokinesis. Proplastids in the giant cells became structurally altered to form tubular complexes. The resistant plants usually responded to the nematodes by a hypersensitivity reaction followed by extensive wall buildup in cells surrounding the infection sites. Nematodes often remained in the necrotic tissues.
Degree
PhD
College and Department
Life Sciences; Plant and Wildlife Sciences
Rights
http://lib.byu.edu/about/copyright/
BYU ScholarsArchive Citation
Tait, Bernard Al, "Light and electron microscopy of resistant and susceptible alfalfa roots infected by meloidogyne hapla" (1974). Theses and Dissertations. 7988.
https://scholarsarchive.byu.edu/etd/7988
Date Submitted
1974-04-01
Document Type
Dissertation
Handle
http://hdl.lib.byu.edu/1877/Letd478
Keywords
Alfalfa, Diseases and pest resistance; Nematodes
Language
English