Abstract
Analysis of hemogobins were conducted on animals of the species Ambystoma tigrinum. Separation of hemoglobin types was performed with a Carboxy-methyl-cellulose column (6 X 50 mm.) and pH gradient (pH 6 thru 8) of .01 M phosphate buffer. The hemoglobins in the effluent were detected and analysed with a micro-flow cell in a Beck-man DB-G spectrophotometer attached to a Photovolt Varicord 43 recorder. Two major components of hemoglobin were observed: larval and adult. These two components varied in concentration with the stage of development of the individual. In addition, it was noted that minor peaks ansd shoulders appeared on each peak wich were consistant and characteristic for each major hemoglobin type.
Degree
MS
College and Department
Life Sciences; Plant and Wildlife Sciences
Rights
http://lib.byu.edu/about/copyright/
BYU ScholarsArchive Citation
Mecham, Harvey Dee, "Changes in hemoglobin during metamorphosis of the Salamander" (1967). Theses and Dissertations. 7820.
https://scholarsarchive.byu.edu/etd/7820
Date Submitted
1967-08-01
Document Type
Thesis
Handle
http://hdl.lib.byu.edu/1877/Letd239
Keywords
Hemoglobin; Salamanders
Language
English