Abstract
Bacillus subtilis can synthesize deoxythymidylate via either of two pathways. The working hypothesis is that the two reactions are very similar, differing only with respect to specificity for the type of folate required as substrate. The folates from B. subtilis were isolated and were determined by gel chromatography to be derivatives of pteroyltriglutamate and pteroyldiglutamate. Kav values for the two folate species are reported. Conjugase treatment revealed no other highly conjugated folates present. It was also observed that mutants deficient in one or the other of the two pathways grow at the same rate as wild-type cells under conditions allowing very rapid growth in the absence of exogenous thymine.
Degree
MS
College and Department
Life Sciences; Plant and Wildlife Sciences
Rights
http://lib.byu.edu/about/copyright/
BYU ScholarsArchive Citation
Hintze, David Nelson, "Folate content of Bacillus subtilis" (1975). Theses and Dissertations. 7778.
https://scholarsarchive.byu.edu/etd/7778
Date Submitted
1975-04-01
Document Type
Thesis
Handle
http://hdl.lib.byu.edu/1877/Letd197
Keywords
Bacteria
Language
English