Abstract
The PstSCAB2 complex serves the dual function of being a phosphate transporter as well as the primary sensor of phosphate for the Pho regulon. PhoU is an integral protein required for the signal from PstSCAB2 to be transmitted to PhoR. Our hypothesis is that conformational changes of PstSCAB2 during the phosphate transport process are the mechanism by which information about environmental phosphate levels are transduced to the cell. Additionally, we propose that direct protein-protein interactions between PhoU and the alternating conformations of PstSCAB2 mediate PhoU interactions with PhoR. By means of genetic and biochemical approaches, we have found substantial evidence supporting both these hypotheses.
Degree
MS
College and Department
Life Sciences; Microbiology and Molecular Biology
Rights
http://lib.byu.edu/about/copyright/
BYU ScholarsArchive Citation
Johns, Kristine Dawn, "Evidences for Protein-Protein Interactions Between PstB and PhoU in the Phosphate Signaling Complex of Escherichia coli" (2013). Theses and Dissertations. 3932.
https://scholarsarchive.byu.edu/etd/3932
Date Submitted
2013-03-15
Document Type
Thesis
Handle
http://hdl.lib.byu.edu/1877/etd6044
Keywords
Pho regulon, two-component, ABC transporter, PstB, PhoU
Language
English