Abstract

This thesis investigates the kinetic modeling of bacteria removal from suspensions using magnetic nanoparticle (MNP) capture and magnetic separation. Functionalized magnetic nanoparticles, especially those coated with polydopamine (pDA), were employed to bind with various bacterial species in phosphate-buffered saline (PBS), apple juice, whole blood, and its components. By analyzing the capture efficiency and kinetics of capture across different bacterial species and mediums, we developed mathematical models describing the capture kinetics of bacteria by the pDA-MNPs. Experimental results demonstrated that pDA-coated MNPs achieved high capture efficiency with certain Gram-positive species like Staphylococcus aureus, and one Gram-negative species, Neisseria perflava; however, capture is less effective with other Gram-negative species such as Escherichia coli and Pseudomonas aeruginosa. Herein, we developed kinetic models, including first-order and multi-compartment models, that were applied to describe the time-dependent capture efficiency, which varies based on the MNP concentration and the suspending medium. The results provide insights into scaling the process for clinical and industrial applications, such as bacterial detection in blood and food safety monitoring.

Degree

MS

College and Department

Ira A. Fulton College of Engineering; Chemical Engineering

Rights

https://lib.byu.edu/about/copyright/

Date Submitted

2024-12-19

Document Type

Thesis

Keywords

Magnetic nanoparticles, polydopamine, bacteria capture, sepsis, iron oxide nanoparticles, capture kinetics, flocculation

Language

english

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Engineering Commons

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