Abstract
Cutthroat trout (Oncorhynchus clarkii) is a salmonid native to Western North America. For almost 200 years, the taxonomy of these species has fluctuated as ichthyologists and fisheries managers alike have tried to understand the diversity seen within these fishes. In the 1950s, Robert Behnke reexamined the cutthroat trout and identified 14 subspecies based on morphological observations, Pleistocene events, and modern geographic ranges. These designations were instrumental in preserving the remaining diversity of cutthroat trout. Over time, molecular techniques (i.e. karyotypes, allozymes, and mtDNA) have largely replicated Behnke's phylogenies. However, these studies have revealed that some of these relationships are consistently weakly supported, resulting in differences in these phylogenies. To further resolve these relationships, we generated de novo transcriptomes for nine cutthroat subspecies, as well as a Bear River Bonneville form and two Colorado River lineages (Blue and Green). We present a phylogeny of these subspecies generated from 30 orthologous genes extracted from our transcriptomes. We confirm many of the relationships identified in previous morphological and molecular studies, as well as discuss significant differences in our phylogeny from these studies.
Degree
MS
College and Department
Life Sciences; Microbiology and Molecular Biology
Rights
https://lib.byu.edu/about/copyright/
BYU ScholarsArchive Citation
Kokkonen, Andrea Leena, "Using de novo Transcriptomes to Decipher the Relationships in Cutthroat Trout Subspecies (Oncorhynchus clarkii)" (2023). Theses and Dissertations. 10525.
https://scholarsarchive.byu.edu/etd/10525
Date Submitted
2023-08-14
Document Type
Thesis
Handle
http://hdl.lib.byu.edu/1877/etd13363
Keywords
cutthroat trout, Oncorhynchus clarkii, transcriptomes
Language
english