Abstract

The PstSCAB2 complex serves the dual function of being a phosphate transporter as well as the primary sensor of phosphate for the Pho regulon. PhoU is an integral protein required for the signal from PstSCAB2 to be transmitted to PhoR. Our hypothesis is that conformational changes of PstSCAB2 during the phosphate transport process are the mechanism by which information about environmental phosphate levels are transduced to the cell. Additionally, we propose that direct protein-protein interactions between PhoU and the alternating conformations of PstSCAB2 mediate PhoU interactions with PhoR. By means of genetic and biochemical approaches, we have found substantial evidence supporting both these hypotheses.

Degree

MS

College and Department

Life Sciences; Microbiology and Molecular Biology

Rights

http://lib.byu.edu/about/copyright/

Date Submitted

2013-03-15

Document Type

Thesis

Handle

http://hdl.lib.byu.edu/1877/etd6044

Keywords

Pho regulon, two-component, ABC transporter, PstB, PhoU

Included in

Microbiology Commons

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