Keywords

Bio-filtration, Plasmid, DNA, Borosilicate, Filters, separation, swinnex, glass filter, ion exchange, affinity, chromotography

Abstract

This research has been completed to determine whether plasmid DNA will bind to a simple borosilicate filter in a reusable filter holder, and whether it can be released in a controlled procedure after a wash has been done to eliminate impurities.

The motivation for this is to help small research groups, both in educational settings as well as in other small research settings be able to study isolated DNA without much of the expense generally associated with purification kits and to remove the limitations on trial numbers imposed by the expense of each individual isolation kit.

Medical research, forensics, and other biological studies often require the examination or multiplication of DNA. In order to examine the DNA, it must be separated from the other cellular components, or lysosomes. Many techniques have been developed by research groups to accomplish the goal of DNA isolation, including through Chromatography. This is any method that uses a stationary phase and a mobile phase. Some types of chromatography include Affinity Chromatography, Size Exclusion Chromatography and Ion Exchange Chromatography.

Document Type

Report

Publication Date

2016-08-12

Permanent URL

http://hdl.lib.byu.edu/1877/3752

Language

English

College

Physical and Mathematical Sciences

Department

Physics and Astronomy

University Standing at Time of Publication

Staff/Researcher

Course

RET 2016

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