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Keywords
histones, nucleosomes, epigenetics, gene expression
Abstract
Chromatin structure and composition play a significant role in regulating gene expression within eukaryotic cells. The fundamental organizational unit of chromatin is the nucleosome, which is composed of ~147 base pairs of DNA wrapped around a histone protein core. Post-translational modifications (PTM’s) to the protruding tails of histone proteins are known to regulate nucleosome occupancy and positioning, which influence chromatin architecture and thus gene expression. However, relatively little is known about the residual effects of histone PTM’s on transcription rate, especially in nucleosomes along the gene body. Here, we demonstrate a method for measuring transcription rate in real-time with a common qPCR machine. This lays the framework for a novel in vivo system in which we will use C. elegans transcriptional machinery to directly study the effects of histone PTM’s on transcription rate, independent of changes to chromatin architecture.
BYU ScholarsArchive Citation
Evans, Taryn; Ramirez, Luke M.S; Bohn, Aaron; Hunter, Jacob; Hodson, Sarah; Ricks, Sarah; Bates, David Ph.D; and Johnson, Steven M. Ph.D., "Laying Framework for a Novel System to Test Effects of Histone Modifications" (2025). Library/Life Sciences Undergraduate Poster Competition 2025. 31.
https://scholarsarchive.byu.edu/library_studentposters_2025/31
Document Type
Book
Publication Date
2025
Language
English
College
Life Sciences
Department
Microbiology and Molecular Biology
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