Files
Download Full Text (594 KB)
Keywords
cancer, DNA, biospecimen, molecular analysis
Abstract
Personalized cancer care requires molecular characterization of neoplasms. While the research community accepts frozen tissues as the gold standard analyte for molecular assays, the source of tissue for all testing of tumor tissue in clinical cancer care comes almost universally from formalin-fixed, paraffin-embedded tissue (FFPE). Specific to genomics assays, numerous studies have shown significant discordance in genetic information obtained from FFPE samples and cryopreserved or flash frozen samples. To explain the discordance between FFPE samples and cryopreserved samples, a head-to-head comparison between FFPE and cryopreserved tissues was performed to analyze the DNA yield, DNA purity and DNA quality in terms of DNA length.
BYU ScholarsArchive Citation
Anderson, DeLaney; Okojie, Jeffrey; Burr, Mackenzie; Worley, Peyton; Parker, Isaac; Davis, Jack; Kearns, Bridger; and Barrott, Jared, "The Cold Hard Truth: Cryopreserved Tissue is Superior to FFPE Tissue in Molecular Analysis" (2024). Library/Life Sciences Undergraduate Poster Competition 2024. 71.
https://scholarsarchive.byu.edu/library_studentposters_2024/71
Document Type
Poster
Publication Date
2024-03-21
Language
English
College
Life Sciences
Department
Neuroscience
Copyright Use Information
https://lib.byu.edu/about/copyright/