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Keywords

Cancer, Antibodies, Biomarker, Thymidine Kinase 1

Abstract

Engineered antibodies used in immunotherapies have been increasingly successful due to their ability to uniquely target cells expressing specific cancer antigens Thymidine Kinase 1 (TK 1 is a DNA salvage enzyme typically found in the cytosol However, in certain types of cancer, TK 1 is surface expressed, making it a unique cancer biomarker By identifying antibodies with a high binding affinity for TK 1 we can target cancer cells expressing TK 1 on their surface Using cell sorting and a yeast display library expressing 109 human single chain antibody fragments, we have isolated ten unique single chain antibodies scFvs that bind to TK 1 Flow cytometry affinity characterization of the 10 clones revealed strong binding affinity in the low nM range Sequencing of the scFvs showed they all had unique complementarity determining regions ( with some similarities among the clones The scFvs were cloned into constructs containing an antibody constant domain and they will be cultured with cancer cell lines expressing TK 1 and evaluated for antibody dependent cellular cytotoxicity ( This research has the potential to target TK 1 cancers and maximize cancer cell death while minimizing harm to healthy cells

Document Type

Poster

Publication Date

2023-03-03

Language

English

College

Life Sciences

Department

Microbiology and Molecular Biology

University Standing at Time of Publication

Junior

Engineering and Characterization of Antibodies Against Cancer Biomarker Thymidine Kinase 1

Included in

Microbiology Commons

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