BYU ScholarsArchive

Title

Sperm DNA Damage Shown To Correlate With DNA Alterations

Authors

Hailey E. Johnson, Brigham Young University - ProvoFollow
Chad Pollard, Brigham Young University - Provo
Timothy Jenkins, Brigham Young University - ProvoFollow

Files

Keywords

DNA Methylation, DNA Damage, Infertility, Hypermethylation, Hypomethylation

Abstract

DNA methylation is a biological process where methyl groups are added to the DNA molecule, changing the physical structure

Sperm DNA methylation has shown to predict pregnancy outcomes for those with infertility

DNA damage can happen through single and double stranded breaks. The Comet and TUNEL assay are two different and common ways to quantify levels of sperm DNA damage.

It is suggested DNA damage might relate with global DNA methylation patterns in sperm genome, however regional differences have been overlooked.

We wanted to assess the relationship between sperm DNA damage and regional DNA methylation, and compare the outcomes of the two different DNA damage assays.

Methylation is described by beta values, which range 0 1 and describe the intensity of methylation at a region in the genome.

DNA Damage was measured by the Comet and TUNEL assay. TUNEL produces a “DFI” score.

BYU ScholarsArchive Citation

Johnson, Hailey E.; Pollard, Chad; and Jenkins, Timothy, "Sperm DNA Damage Shown To Correlate With DNA Alterations" (2023). Library/Life Sciences Undergraduate Poster Competition 2023. 14.
https://scholarsarchive.byu.edu/library_studentposters_2023/14

Document Type

Poster

Publication Date

2023-02-22

Language

English

College

Life Sciences

Department

Biology

University Standing at Time of Publication

Sophomore

Copyright Use Information

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