The Influence of Staphylococcus Aureus Biofilm associated Gene Mutations on Biofilm Composition

Title

The Influence of Staphylococcus Aureus Biofilm associated Gene Mutations on Biofilm Composition

Files

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Keywords

MRSA, biofilm, biofilm composition, Staphylococcus aureus

Abstract

Video Presentation 2nd Place Award Winner

Staphylococcus aureus (biofilms are serious impediments to immune defenses and antibiotics making them a major factor in SA infections. Such infections can be highly lethal despite current treatments, presenting a major challenge to the healthcare industry. Previous genetic screenings of SA have revealed several genes that may be associated with biofilm formation. While many of these genes have been studied, little research has been done to examine how mutations of said genes impact biofilm composition. As several therapeutic options for treating mature SA biofilms require an understanding of the biofilm’s composition, an effort to better comprehend how the genes influence that composition is critical to improving current treatments and developing new ones.

In this project, we are conducting a study the biofilm phenotypes of the Je 2 strain of SA with mutations in common biofilm associated genes. By comparing the biofilm mass and composition of the wild type Je 2 strain to strains containing mutated biofilm associated genes, we hope to uncover the impact of each mutation on the composition of the biofilm matrix. To make comparisons between mutant biofilms and wild type biofilms, variants of a crystal violet assay are used to determine and characterize biofilm composition.

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Document Type

Video

Publication Date

2020-4

Language

English

College

Life Sciences

Department

Microbiology and Molecular Biology

University Standing at Time of Publication

Senior

The Influence of Staphylococcus Aureus Biofilm associated Gene Mutations on Biofilm Composition

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