Tissue thiamin diphosphate was assayed enzymatically utilizing purified brewer's yeast apotransketolase. With this assay ten uumoles of thiamin diphosphate could be detected. Total thiamin was fluorometrically measured. For one to three days rats were placed under conditions of stress, consisting of loud noise treatment, emotional stress, deprivation of paradoxical sleep, and swimming to exhaustion, respectively. The body weight was markedly decreased at the end of the respective experimental periods except with the loud noise treated group, compared with the corresponding controls. Following two to three such consecutive treatments, the adrenal weight also showed a significant increase. Thiamin diphosphate and total thiamin in liver was significantly decreased after one day's exposure to loud noise. On the other hand, thiamin diphosphate and total thiamin in brain and liver were not significantly affected by the other treatments tried in this study .
College and Department
Chemistry and Biochemistry
BYU ScholarsArchive Citation
Park, Dong Hwa, "The effects of various treatments on thiamin diphosphate and total thiamin levels of rat tissues" (1970). Theses and Dissertations. 8329.
Thiamin pyrophosphate, Rats, Physiology