Title

The purification and characterization of cytochrome b₅ from porcine kidney

Abstract

A soluble form of cytochrome b_5, with a 413/280 nm ratio of nearly unity, has been isolated and purified from porcine kidney using fractional precipitation, ion exchange resins, and gel filtration procedures. The cytochrome b_5 shows heat stability up to 50° and is stable in the pH range 6.0 to 8.5. The oxidized protein exhibits an absorption maximum at 413 nm, while the reduced form shows three peaks: 423, 526, 556 nm. Molecular weight determinations have given variable results, suggesting the possibility of a polymer composed of four and eight units of a 13,500 molecular weight protomer. This study has used no lipases, proteases, or detergents in the solubilization of the cytochrome, and the possibility exists that the cytochrome found represents newly formed cytochrome b_5 which is as yet unattached to the microsomes, and which reflects the form found in intact cells.

Degree

MS

College and Department

Chemistry and Biochemistry

Rights

http://lib.byu.edu/about/copyright/

Date Submitted

1973-04-01

Document Type

Thesis

Handle

http://hdl.lib.byu.edu/1877/Letd597

Keywords

Cytochrome c., Swine, Physiology, Kidneys

Language

English

This document is currently not available here.

Share

COinS