Ethionine-resistant mutans of Plectonema boryanum have been isolated and partially characterized. The mutant strains exhibit at least 100-fold increase in resistance to L-ethionine. The nature of the change which confers ethionine resistance is not known, but appears to result from the inability of the resistant strains to incorporate ethionine into acid-precipitable material. Initially, ethionine has no effect on the growth rate of the wild-type or the resistant strains, but several hours of exposure to the analog at a concentration greater than 0.5 μg/ml causes a marked inhibition of growth and bleaching in the wild-type cells. The onset of bleaching precedes observable growth inhibition by three to five hours. The mutants are stable and have other properties which make them potentially useful as markers in genetic studies.
College and Department
Plant and Wildlife Sciences
BYU ScholarsArchive Citation
Hentschel, Wayne M., "Ethionine-resistant mutants of the filamentous blue-green alga Plectonema boryanum /|cby Wayne M. Hentsche" (1977). Theses and Dissertations. 8070.