Abstract

(1,3;1,4)-β- D-glucan (β-glucan) is a plant cell wall hemicellulose and a main component of endosperm cell walls. The Cellulose Synthase F family of genes is involved in the synthesis of β-glucan. In this study full-length genomic sequences of CslF6 were obtained from multiple Avena species. Three unique alleles were found in each A. sativa line. Comparisons of these alleles to diploid Avena species allowed for identification of the genomic origin of each allele. The A and D genome alleles had identical amino acid sequences while the C-genome had 13 different amino acids. Global expression of CslF6 was completed at three developmental time point and three tissue types. RNAseq technology was utilized to determine genome specific expression patterns. Differential expression of genome specific-copies of CslF6 was found at all time points tested. Lower levels of C-genome expression of CslF6 were associated with increased levels of B-glucan.

Degree

College and Department

Life Sciences; Plant and Wildlife Sciences

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