Abstract
Centromere binding factor 1 (Cbf1) is a transcription factor that controls the transcription of many genes involved in cellular respiration and lipid biogenesis and, as such, has been associated with hypolipidemia in humans. It is a known substrate for PAS kinase, which phosphorylates Cbf1 and alters its activity. Our hypothesis is that this phosphorylation affects the genes it regulates and the DNA motifs it binds to, perhaps due to different transcription complexes being formed. In this study, we conduct a chromatin immunoprecipitation in Saccharomyces cerevisiae to determine what genes and DNA motifs Cbf1 binds to in its wild type versus phosphosite mutant forms. We discovered several motifs that may be specific to each Cbf1 form, however further evidence is necessary. We were able to identify five motifs in reads associated with phosphosite Cbf1, while reads associated with wild type Cbf1 had 16 motifs, with no overlap between the motifs found from the two forms. This may be due to phosphorylated Cbf1 having more binding partners. Cbf1 regulated genes and possible transcription complex binding partners are proposed.
Degree
MS
College and Department
Life Sciences; Microbiology and Molecular Biology
Rights
https://lib.byu.edu/about/copyright/
BYU ScholarsArchive Citation
Ellsworth, Spencer, "Characterization of Cellular Metabolism Regulation by the Transcription Factor Centromere Binding Factor 1 (Cbf1)" (2024). Theses and Dissertations. 10337.
https://scholarsarchive.byu.edu/etd/10337
Date Submitted
2024-04-16
Document Type
Thesis
Handle
http://hdl.lib.byu.edu/1877/etd13175
Keywords
Cbf1, PAS kinase, cellular respiration, lipogenesis, hyperlipidemia, yeast
Language
english