Cell Invasion, Placenta, PKM2, Trophoblast
The Pyruvate kinase isozymes M2 (PKM2) protein is a metabolic enzyme that regulates the final step of glycolysis. This enzyme is present in highly proliferating cells such as those present in embryonic tissues, cancer and in the placental trophoblast. We recently studied PKM2 protein in the human placenta at term. Our results demonstrated upregulated placental PKM2 during preeclampsia (PE) where trophoblast invasion is decreased and hypoxia is present. Our objective was to determine the cytosolic and nuclear PKM2 expression pattern and determine PKM2 regulation of trophoblast cell invasion. Trophoblast cells were cultured in normoxic and hypoxic conditions and real time cell invasion was determined during activation (Fructose-6-bisphosphate) or inhibition (Shikonin) of PKM2. Treated cells were lysed for western blot analysis of PKM2. During normoxia, we observed: 1) 2-fold increase (p
BYU ScholarsArchive Citation
Mejia, Juan; Hall, Parker; Hirschi, Kelsey; Reynolds, Paul R.; and Arroyo, Juan, "Regulation of Trophoblast Invasion by Pyruvate Kinase Isozyme M2 (PKM2)" (2018). Student Publications. 234.
Physiology and Developmental Biology
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