Discovering Diet-Dependent Changes in Amino Acid Metabolism

Authors

Benjamin Driggs, Brigham Young UniversityFollow
Coleman Nielsen, Brigham Young University
J. C. Price, Brigham Young UniversityFollow

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Keywords

kinetic proteomics, metabolism, isotopic labeling, dietary restrictions

Abstract

The Price Lab ran a mouse diet experiment to see if we could detect changes in amino acid metabolism according to diet. The diets used were low protein ad libitum (LP AL), low protein CR (LP CR), high protein ad libitum (HP AL), and high protein CR (HP CR). Multiple individuals were randomly assigned to each diet. Extracting D2O labelled samples across several time points allows us to use mass spectrometry to measure both protein concentration and turnover (figure 1) (1). These are measured by analyzing the shift in isotopic peaks (2). Using these changes in isotopic peaks we can also calculate n-values (see figure 2 below), which are metabolic indicators for the associated molecule.

BYU ScholarsArchive Citation

Driggs, Benjamin; Nielsen, Coleman; and Price, J. C., "Discovering Diet-Dependent Changes in Amino Acid Metabolism" (2025). Library/Life Sciences Undergraduate Poster Competition 2025. 61.
https://scholarsarchive.byu.edu/library_studentposters_2025/61

Document Type

Article

Publication Date

2025

Language

English

College

Life Sciences

University Standing at Time of Publication

Senior

Copyright Use Information

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