Selection of high-affinity scFvs against tumor antigen HPRT for use in cancer immunotherapies
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cancer, tumor, immunotherapy, CAR T cell, yeast display
Hypoxanthine guanine phosphoribosyltransferase (HPRT) is a DNA salvage pathway repair enzyme found in the cytoplasm. Recent studies show that HPRT is upregulated and surface expressed in certain cancers, making HPRT a potential cancer biomarker. Our goal is to identify high affinity antibodies against HPRT for use in cancer immunotherapies. We isolated HPRT antibodies from a yeast display system and characterized them to ensure they are unique, bind with high affinity, and are specific to cancer cells. They will then be cloned into immunotherapy constructs and assessed for cytotoxicity. Our research to identify high affinity antibodies against HPRT could lead to development of effective immunotherapeutic treatments for cancer.
BYU ScholarsArchive Citation
Johnson, Abigail; Hanie, Christopher; Whitley, Kiara; Meier, Stella; Lindsay, Hunter; Townsend, MIchelle; O'Neill, K. L.; and Weber, Scott, "Selection of high-affinity scFvs against tumor antigen HPRT for use in cancer immunotherapies" (2022). Library/Life Sciences Undergraduate Poster Competition 2022. 3.
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