Novel HIV-1 Treatment through CRISPR Technology utilizing dual guide RNA Novel HIV-1 Treatment through CRISPR Technology utilizing dual guide RNA
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Utilizing dual guide RNA to prevent the onset of AIDS
We propose a treatment for HIV-1 by developing a system that gives infected cells the ability to recognize and destroy the integrated HIV-1 genome. Previous studies have shown that through the guide RNA directed CRISPR-Cas9 gene editing system, complete virus inactivation can be achieved in cultures T cells1. HIV has been known to escape a single antiviral gRNA by mutation of the specific sequence that is targeted1. This is why, in our approach, we use a dual guide RNA system that targets the viruses highly conserved TatRev and Gag1 genes. It has been shown that this combination of combinatorial gRNA completely sterilizes HIV-1 in a T cell infection1. Our goal is to take this dual gRNA directed CRISPR-Cas9 system a step further with our humanized mouse model. Engraftment of human immune cells into immunodeficient mice has developed as a cutting-edge technology that few researchers in the world know how to use. We are using the methods of transfection and transduction to give human hematopoietic stem cells the dual gRNA CRISPR-Cas9 system to recognize the integrated HIV-1 genome and cut it out of the human genome. We plan to inject these HSC’s into immunodeficient mice, and infect the mouse model with HIV-1. We expect to see complete inactivation and sterilization of HIV-1, preventing the virus from causing disease.
BYU ScholarsArchive Citation
Magaoay, Daniel; Tidwell, Mary; and Berges, Bradford K. Ph.D., "Novel HIV-1 Treatment through CRISPR Technology utilizing dual guide RNA Novel HIV-1 Treatment through CRISPR Technology utilizing dual guide RNA" (2021). Library/Life Sciences Undergraduate Poster Competition 2021. 15.
Microbiology and Molecular Biology
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