Illuminating novel genes for Escherichia coli growth in varying phosphate conditions using TnSeq and Illumina Sequencing

Authors

Tanner Robinson, Brigham Young University
Dr. William R. McCleary, Brigham Young University

Keywords

novel genes, Escherichia coli growth, phosphate conditions, TnSeq and Illumina sequencing

College

Life Sciences

Department

Microbiology and Molecular Biology

Abstract

The purpose of my project is to identify novel genes in the genome of Escherichia coli ( E coli) associated with phosphate homeostasis under high phosphate conditions. The previous efforts that have been employed were great and innovative for their time, but both lacked the technology currently available, and focused solely on lowphosphate response. Other labs have used variations of the TnSeq protocol that I have, to address a variety of questions in many organisms, with great success (Subashchandrabose et al., 2013).

Recommended Citation

Robinson, Tanner and McCleary, Dr. William R. (2017) "Illuminating novel genes for Escherichia coli growth in varying phosphate conditions using TnSeq and Illumina Sequencing," Journal of Undergraduate Research: Vol. 2017: Iss. 1, Article 216.
Available at: https://scholarsarchive.byu.edu/jur/vol2017/iss1/216