Journal of Undergraduate Research
Keywords
small heat shock proteins, CryAB, HspB2, cardiac robustness
College
Life Sciences
Department
Microbiology and Molecular Biology
Abstract
Evaluation of how well the academic objectives of the proposal were met
- Specific Aim 1: Perform yeast two-hybrid screens on wild type CryAB, R120G CryAB, and HspB2 in order to create a protein-protein interaction network and identify potential substrates.
- Achievements: Large-scale yeast-two hybrid screens were performed on wild type CryAB, R120G CryAB and HspB2 in order to identify their protein-protein interaction networks and to identify putative substrates.
- Specific Aim 2: Validate the protein-protein interaction network via Y2H dependency tests (A), affinity purification from mammalian cells (B) and chaperone activity assays (C).
- Achievements: Y2H dependency tests were performed for all three proteins (CryAB, R120G and HspB2), and a subset of the interactomes was verified through both in vitro (B) and in vivo chaperone assays (C).
Recommended Citation
Grose, Julianne H.
(2015)
"Elucidating a Precise Role for the Small Heat Shock Proteins CryAB and HspB2 in Cardiac Robustness,"
Journal of Undergraduate Research: Vol. 2015:
Iss.
1, Article 245.
Available at:
https://scholarsarchive.byu.edu/jur/vol2015/iss1/245