•  
  •  
 

Journal of Undergraduate Research

Keywords

culture, isolation, embryonic stem cells, porcine blatocysts, ES

College

Life Sciences

Department

Plant and Wildlife Sciences

Abstract

Embryonic stem (ES) cells are obtained through the culture of undifferentiated cells of embryos in early devel-opment. Because embryonic cells will undergo differentiation and development when placed in standard culture conditions, a media which contains differentiation inhibitors must be used in order for successful development of ES cells. ES cells are capable of becoming any cell type in the body when removed from the influence of differentiation inhibitors, making ES cells a potential agent for gene transfer and livestock cloning. ES cells are therefore an invaluable research tool, providing both a better understanding of genetics as well as providing genetically superior animals. The successful culture of embryonic stem cells depends upon accurate and reliable isolation techniques. The purpose of this research was to determine the optimum method for isolation and culture of ES cells and also to determine which differentiation inhibitor works most effectively. Embryos were collected and cultured for the purpose of both perfecting current techniques and studying which differentiation inhibiting media supported maximum growth.

Download

Share

COinS