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Journal of Undergraduate Research

Keywords

phylogenetic, crayfish, astacin, Astacus astacus, PCR, Polymerase Chain Reaction

College

Life Sciences

Department

Physiology and Developmental Biology

Abstract

Astacin is a zinc-endopeptidase, originally described as a unique enzyme and first sequenced from the crayfish Astacus astacus (Geier 97). This project aimed to gather several Astacin gene sequences from multiple taxa of crayfish and to submit them to phylogenetic analysis using PAUP (Swofford 98). PAUP would then generate trees displaying the most probable evolutionary relationships among taxa based on variation in the Astacin gene sequences.

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