The Potential for Grape Seed Extract as an Antioxidant in Human Cells

Authors

Mark J. Eliason, Brigham Young University
Dr. Kim O'Neill, Brigham Young University

Keywords

grape seed extract, antioxidant, human cells, GSE, electrophoresis assay

College

Life Sciences

Department

Microbiology and Molecular Biology

Abstract

The single-cell gel electrophoresis assay or “Comet Assay” is a novel test which can be used to empirically ascertain the degree of damage sustained by the DNA of individual cells when exposed to cytotoxic chemicals. In this experiment a known cell line was grown in varying concentrations of grape seed extract (GSE), a reputed antioxidant, and then exposed to hydrogen peroxide, an oxidant, known to cause breaks in the DNA. When the comet assay was subsequently run the effect that the varying concentrations of grape seed extract had in protecting the cells from damage could be quantified.

Recommended Citation

Eliason, Mark J. and O'Neill, Dr. Kim (2014) "The Potential for Grape Seed Extract as an Antioxidant in Human Cells," Journal of Undergraduate Research: Vol. 2014: Iss. 1, Article 847.
Available at: https://scholarsarchive.byu.edu/jur/vol2014/iss1/847