Journal of Undergraduate Research
Keywords
genotoxicity, 1.4-Dioxane, DX, single cell gel, DNA
College
Life Sciences
Department
Microbiology and Molecular Biology
Abstract
1.4-Dioxane (DX), a common ingredient in solvents for oils, waxes and many other organic compounds, has an undetermined genotoxicity. It has been reported to be both a nongenotoxic (Salmonella and Cl-IO chromosome aberration assays) and a genotoxic compound (1). Previous experiments carried out indicate that Sherman strain and Sprague-Dawley rats receiving 0.1% to 1% DX in drinking water develop hepatic/renal damage and hepatocellular and nasal carcinoma respectively (4,7). In this preliminary study, the single cell gel assay was used to determine possible DNA damage caused by DX. Human Raji Burkitt’s lymphoma cells were grown In the presence of various concentrations (50-300 mM) of DX. Cells exposed to DX were analyzed for DNA damage after 24, 48 and 72 hours. DNA damage was exhibited in the 100 mM range, about equivalent to 1.0% DX in the drinking water of the animals that developed carcinomas. The Single Cell Gel Assay is proving to be more sensitive to genotoxic agents than the Salmonella and Cl-IO chromosome aberration assays.
Recommended Citation
Harris, Edward
(2014)
"THE GENOTOXICITV OF 1.4-DIOXANE USING THE SINGLE CELL GEL,"
Journal of Undergraduate Research: Vol. 2014:
Iss.
1, Article 807.
Available at:
https://scholarsarchive.byu.edu/jur/vol2014/iss1/807