Preparation of Green Fluorescent Protein Constructs for the Determination of the Mechanism of the Excision of a Novel Intron

Authors

John Daniel Clinger, Brigham Young University
Dr. Daniel L. Simmons, Brigham Young University

Keywords

green fluorescent protein, GFP, excision, novel intron

College

Physical and Mathematical Sciences

Department

Chemistry and Biochemistry

Abstract

Recent advances have greatly increased the utility of bioluminescence as a qualitative and quantitative tool in scientific research. The genes encoding fluorescent proteins in several organisms have been identified, cloned, modified, and inserted into several different vectors; thus allowing them to be used in a variety of diagnostic techniques. One such protein, isolated from aequorea victoria, is green fluorescent protein (GFP). Since GFP is encoded by a small gene, 720 base pairs, and fluoresces brightly when irradiated by ultraviolet light; it was seen as good candidate for the study of a novel pre-mRNA splicing mechanism.

Recommended Citation

Clinger, John Daniel and Simmons, Dr. Daniel L. (2014) "Preparation of Green Fluorescent Protein Constructs for the Determination of the Mechanism of the Excision of a Novel Intron," Journal of Undergraduate Research: Vol. 2014: Iss. 1, Article 1148.
Available at: https://scholarsarchive.byu.edu/jur/vol2014/iss1/1148