Identification of The Regulatory Elements Responsible For The Control of PremRna Splicing of Intron-1 in Chicken Cox-2

Authors

Daniel L. Simmons, Brigham Young University
Dr. Orlan Kenneth Macdonald, Brigham Young University

Keywords

regulatory elements, PremRna splicing, intron-1, chicken COX-2

College

Physical and Mathematical Sciences

Department

Chemistry and Biochemistry

Abstract

In order to create a unique clone of chicken COX-2 it was necessary to insert a synthetic DNA tag into the full-length version of the COX-2 cDNA. First, a 21-nucleotide oligo and its complement were produced using a DNA synthesizer. The oligos were purified, phosphorylated and then annealed.

Recommended Citation

Simmons, Daniel L. and Macdonald, Dr. Orlan Kenneth (2014) "Identification of The Regulatory Elements Responsible For The Control of PremRna Splicing of Intron-1 in Chicken Cox-2," Journal of Undergraduate Research: Vol. 2014: Iss. 1, Article 1141.
Available at: https://scholarsarchive.byu.edu/jur/vol2014/iss1/1141