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Journal of Undergraduate Research

Keywords

tmRNA, SmpB, rescue complex binds, stalled ribosomes

College

Physical and Mathematical Sciences

Department

Chemistry and Biochemistry

Abstract

Ribosomes are large complexes made up of ribosomal RNA and proteins and are the site of protein synthesis. However, ribosomes occasionally run into problems during this process and will stall during translation. Fortunately, two molecules known as tmRNA and SmpB are made by cells in order to free stalled ribosomes. We sytematically mutated G517, C518, C519 and A520 in the ribosome to see if binding fidelity decreases, and to see if these bases affect the activity of tmRNA/SmpB in ribosomes during ribosome rescue. This assay however, gave us no informative results because we were only looking at endpoints of its rescue.

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