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Journal of Undergraduate Research

Keywords

phosphate, ferritin iron, deposition kinetics

College

Physical and Mathematical Sciences

Department

Chemistry and Biochemistry

Abstract

I performed a ferritin iron reconstitution experiment, making 10 sequential additions of 10 ferrous ions per ferritin molecule at 10- minute intervals (see Figure 1). Monitoring the reaction at 375 nm, I observed a fast initial absorbance change representing iron oxidation. After the initial oxidation, I observed a gradual increase in absorbance that was complete in 10 minutes. I fit the plot of the change in absorbance versus time for each addition of iron to a double exponential equation (dA/dt = -A1ekt + -A2e-kt + c). The double exponential consisted of two negative terms. k1 has a relatively large value and represents a fast initial reaction. k2 is small and represents a slower sequential reaction.

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