Journal of Undergraduate Research
Keywords
Na, K-Atpase, ocular lens, subunit isoforms, SDS-PAGE
College
Physical and Mathematical Sciences
Department
Chemistry and Biochemistry
Abstract
The Na,K-ATPase is a membrane-bound enzyme found in all cells. This enzyme is composed of an a subunit, which is responsible for the enzyme’s catalytic activity, and a b subunit, which assists in the binding of ions. The three main isoforms of the a subunit are designated a1, a2, and a3. The Na,K-ATPase is responsible for maintaining the proper balance of sodium and potassium ions in the intracellular and extracellular fluids Inhibition or decreased activity of the Na,K-ATPase enzyme has been implicated in the development of cataracts. Without the proper ionic balance, lens cells may be damaged and experience protein leakage, which then leads to the formation of opaque plaques, or cataracts. Our objective was to first determine the distribution of the isoforms in the ocular lens and then to investigate their possible hormonal regulation in vitro.
Recommended Citation
Lunceford, Adam L. and Graves, Dr. Steven W.
(2013)
"Distribution and Regulation of Na,K-Atpase in Ocular Lens,"
Journal of Undergraduate Research: Vol. 2013:
Iss.
1, Article 2607.
Available at:
https://scholarsarchive.byu.edu/jur/vol2013/iss1/2607