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Journal of Undergraduate Research

Keywords

phosphoester bond stabilization, enhanced CID phosphoproteomics

College

Physical and Mathematical Sciences

Department

Chemistry and Biochemistry

Abstract

Proteomics uses highly sensitive mass spectrometers to measure peptide fragment masses from enzymatically digested proteins. Measuring the mass of the peptide and fragments produced in the mass spectrometer allows for identification of its parent protein. Phosphoproteomics analyzes peptides that have been phosphorylated. Phosphoproteomics identifies phosphorylation sites, network relationships between proteins, and how cells respond to changes in their environment. Mass spectrometers typically ionize peptides to carry positive charges. This charge aids to draw molecules into the instrument. Phosphate modifications reduce charge on their molecules, reducing the positive charge on phosphopeptides, creating neutral, or negatively charged species. The lack of charge on these species results in a loss of detection.1-2

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