Journal of Undergraduate Research
Keywords
solid-phase extract, protein biomarkers, disease, blood serum
College
Physical and Mathematical Sciences
Department
Chemistry and Biochemistry
Abstract
Biomarkers in blood serum are the keys needed for the early detection of several types of cancer, complications of pregnancy, and potentially many other diseases, to allow time for successful treatment. Blood serum is ideal because itp comes in contact with all parts of the body, its sampling is minimally invasive, and it is easily prepared with high reproducibility. There is a problem, however, that prevents the development and wide-scale utilization of this treasure chest of biological information. Pure blood serum is complex and cluttered with high-abundance non-target proteins which makes examination of the low-abundance and low-molecular weight potential biomarkers extremely difficult. Current protocol for blood serum preparation relies largely on incomplete and ineffective methods, making biomarker proteomics insufficiently reliable and cost effective for clinical application. The project which was carried out focused on the accuracy, reproducibility, simplicity, and speed of sample preparation in order to make biomarker analysis viable for wide scale application.
Recommended Citation
McLean, Peter F. and Thulin, Dr Craig
(2013)
"Use of Solid-Phase Extraction to Enrich Protein Biomarkers of Disease from Blood Serum,"
Journal of Undergraduate Research: Vol. 2013:
Iss.
1, Article 2553.
Available at:
https://scholarsarchive.byu.edu/jur/vol2013/iss1/2553