Journal of Undergraduate Research
Keywords
nanoinjection, DNA, stand-alone lance, egg cells, trauma
College
Ira A. Fulton College of Engineering and Technology
Department
Mechanical Engineering
Abstract
Technologies for injecting DNA into egg cells have greatly facilitated genetic research. Microinjection is a current standard for this purpose. It uses a hollow glass needle to inject a solution containing DNA into cells. Although widely used, microinjection has several significant challenges. First, the DNA can be damaged when forced through the small opening of the needle. Second, the cell can die from the physical trauma caused by the microinjection needle piercing the cell membrane. Third, the cell can split open because of the increased internal pressure caused by injecting fluid into the cell. Brigham Young University has developed nanoinjection as an alternative approach for DNA injection into cells. The basic description of nanoinjection is illustrated in Figure 1.
Recommended Citation
Fernelius, Mark and Howell, Dr. Larry
(2013)
"Stand-Alone Lance for Nanoinjection of DNA,"
Journal of Undergraduate Research: Vol. 2013:
Iss.
1, Article 2016.
Available at:
https://scholarsarchive.byu.edu/jur/vol2013/iss1/2016