Journal of Undergraduate Research
Keywords
cell-free protein synthesis, extract preparation methods
College
Ira A. Fulton College of Engineering and Technology
Department
Chemical Engineering
Abstract
My ORCA project proposed to test and optimize various low capital cost cell lysis methods, while leaving the internal cell machinery intact and viable for cell-free protein synthesis research. Our cells of interest were a strain of the bacterium E. coli, and their cellular machinery consists of the chemicals and enzymes they use in their every-day life processes, including (and of particular interest to us) the synthesis of proteins. These proteins have an almost limitless number of potential applications in medicine, food services, and chemical production, so the various methods of protein synthesis are of significant importance, both financially, and for the improvement of quality of human life. Cell-free extract preparation is a preliminary step in cell- free protein synthesis; our methods of extract preparation and optimization are detailed below.
Recommended Citation
Holland, Troy and Bundy, Dr. Brad
(2013)
"Cell-free Protein Synthesis Extract Preparation Methods,"
Journal of Undergraduate Research: Vol. 2013:
Iss.
1, Article 1786.
Available at:
https://scholarsarchive.byu.edu/jur/vol2013/iss1/1786