Assessment of Merocyanine Subpopulations in DPPC Vesicles using Anisotropy and Lifetime Measurements

Authors

Hannabeth Franchino, Brigham Young University
Dr. John Bell, Brigham Young University

Keywords

dipalmityolphosphadtidylcholine, DPPC, merocyanine 540, dimer fluorescence

College

Life Sciences

Department

Physiology and Developmental Biology

Abstract

This study investigated the effects of lipid phase on monomer and dimer fluorescence of merocyanine 540. Emission and anisotropy spectra were assessed at multiple temperatures covering all four lamellar phases of pure dipalmityolphosphadtidylcholine. The probe segregates in the bilayer into two populations: monomers (emission maximum~585 nm) and dimers (emission maximum~621 nm).

Recommended Citation

Franchino, Hannabeth and Bell, Dr. John (2013) "Assessment of Merocyanine Subpopulations in DPPC Vesicles using Anisotropy and Lifetime Measurements," Journal of Undergraduate Research: Vol. 2013: Iss. 1, Article 1470.
Available at: https://scholarsarchive.byu.edu/jur/vol2013/iss1/1470