Journal of Undergraduate Research
Keywords
apoptotic cell membrane, cytotoxic, chemotherapeutic, S49 lymphoma, confocal microscopy
College
Life Sciences
Department
Physiology and Developmental Biology
Abstract
This project studied and documented the membrane changes of cancer cells undergoing the complex cellular process apoptosis, or programmed cell suicide. More specifically, the purpose of this project was to achieve a visual comparison between S49 mouse lymphoma cells experiencing apoptosis induced by a variety of cytotoxic and chemotherapeutic drugs. This comparison was to be accomplished through cell imaging obtained by merocyanine (MC) 540 fluorescence confocal microscopy. Imaging accomplished in this project is an extension of ongoing research conducted by Dr. Bell and fellow colleagues concerning the action of the secretory phospholipase A2 (sPLA2) enzyme with respect to the effects of various chemotherapeutic drugs on lymphoma cell membranes. This project, along with the associated research concerning sPLA2, is intended to inform the scientific community of the potential implications of chemotherapeutic agents that promote apoptosis in cancer cells and why certain cell populations might exhibit resistance to sPLA2 despite treatment with such drugs. Answering these two questions relies on a sound, precise understanding of the process of programmed cell death that this project aimed to increase.
Recommended Citation
Yeung, Celestine and Bell, Dr. John
(2013)
"Comparative Imaging of Apoptotic Cell Membrane Morphology as Induced by Cytotoxic and Chemotherapeutic in S49 Lymphoma Cells by Confocal Microscopy,"
Journal of Undergraduate Research: Vol. 2013:
Iss.
1, Article 1466.
Available at:
https://scholarsarchive.byu.edu/jur/vol2013/iss1/1466