Journal of Undergraduate Research
Keywords
LNCaP, NF-kappaB, prostate cancer cells
College
Life Sciences
Department
Physiology and Developmental Biology
Abstract
We have previously shown that treatment of LNCaP human prostate cancer cells with a serum-achievable concentration of a biologically relevant form of selenium (Se) dramatically reduces binding of the transcription factor NF-kappaB to its DNA response element, and reduces by half the transcription rates and steady state mRNA levels for anti-apoptotic and other NF-kappaB regulated genes. However, the activation of NF-kappaB is a multistep process (See Figure 1). It is possible that Se could affect various steps in that process ultimately resulting in a decreased binding to DNA. The purpose of this work was to identify those steps in the NF-kappaB activation process affected by Se in order to identify the mechanism(s) by which Se inhibits binding of NF-kappaB to its DNA response element in LNCaP cells.
Recommended Citation
Barzee, Legg
(2013)
"Untitled,"
Journal of Undergraduate Research: Vol. 2013:
Iss.
1, Article 1408.
Available at:
https://scholarsarchive.byu.edu/jur/vol2013/iss1/1408